Hblv-mcherry-puro

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WebpLVX-IRES-mCherry Lentiviral bicistronic expression vector for constitutively co-expressing a protein of interest together with mCherry. Sequence Author: Clontech (TaKaRa) Open in SnapGene Try SnapGene for Free Download Plasmid Download SnapGene Viewer Explore Over 2.7k Plasmids: Viral Expression & Packaging Vectors More Plasmid Sets WebApr 9, 2024 · VectorBuilder offers lentivirus packaging services with superior quality. We have developed a series of proprietary technologies and reagents that have greatly improved recombinant lentivirus production protocols in terms of titer, purity, viability and consistency, especially for the third-generation lentiviral vector systems used in our ...

Downregulating PDPK1 and taking phillyrin as PDPK1-targeting …

WebpLVX-IRES-Puro Sequence and Map pLVX-IRES-Puro Lentiviral vector for bicistronic expression of a gene together with a puromycin resistance marker. Sequence Author: Clontech (TaKaRa) Open in SnapGene Try SnapGene for Free Download Plasmid Download SnapGene Viewer Explore Over 2.7k Plasmids: Viral Expression & Packaging … WebpCDH-EF1-MCS-T2A-Puro EF1 pCDH-EF1-MCS-T2A-copGFP EF1 Medium robust in most cell types, including primary differentiated cells pCDH-MCS-T2A-Puro-MSCV MSCV pCDH-MCS-T2A-copGFP-MSCV MSCV High hematopoietic / stem cell lines Single Promoter pCDH-CMV-MCS CMV pCDH-CMV-MCSr CMV pCDH-CMV-MCS-EF1-Puro CMV Dual … pictures and names of orchids

Lentiviral vector systems with an EF-1 alpha promoter

WebApr 10, 2024 · Puro $159.00 View Vector pAAV[Exp]-EFS>hyPBase Mammalian Gene Expression AAV Vector ... [Exp]-mCherry-CMV>hyPBase WebDec 3, 2024 · To construct short hairpin RNAs (shRNAs) targeting circ-TNPO3 or SNAIL, the shRNA sequences targeting the back-splicing junction of circ-TNPO3 were ligated into the HBLV-GFP-Puro lentiviral vector (Han Biomedical, Shanghai, China), and the shRNA sequences targeting mRNA of SNAIL were ligated into the HBLV-mcherry-BSD lentiviral … WebMammalian Expression, Lentiviral Growth in Bacteria Bacterial Resistance (s) Ampicillin, 100 μg/mL Growth Temperature 37°C Growth Strain (s) NEB Stable Copy number High Copy Gene/Insert Gene/Insert name mCherry Species Synthetic Insert Size (bp) 711 Mutation no Cloning Information Cloning method Restriction Enzyme top golf fredericksburg

Inhibiting PDPK1 and the phosphorylation of PDPK1(ser241) …

Hblv-mcherry-puro

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WebOct 30, 2009 · pLVX-mCherry-N1 is an HIV-1-based, lentiviral expression vector that allows you to express your . gene of interest fused to mCherry, a mutant fluorescent protein derived from the tetrameric . Discosoma sp. red fluorescent protein, DsRed (1). The excitation and emission maxima of the native mCherry protein are 587 nm and 610 nm, … WebpLV-mCherry (Plasmid #36084) Print Enlarge View all sequences Purpose (Empty Backbone) 3rd generation lentiviral plasmid expressing mCherry Depositing Lab Pantelis Tsoulfas Publication Tsoulfas Lab plasmids …

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WebMay 3, 2016 · The vector map of pHBLV-CMVIE-IRES-puro. Cite. Download (700.34 kB) figure. posted on 2016-05-03, 02:08 authored by Dongling Chen, Zhe Jin, Jingjing … WebJan 1, 2024 · Introduction. Nonalcoholic fatty liver disease (NAFLD), characterized by excess hepatic fat accumulation, inflammation and oxidative stress, has become a burgeoning worldwide epidemic liver disease, affecting approximately one-quarter of the entire global population (Diehl and Day, 2024).Moreover, NAFLD not only encompasses …

WebpLVX-EF1α-mCherry-C1 is a lentiviral expression vector that can be used to generate high-titer lentivirus for transducing virtually any dividing or nondividing mammalian cell type, including primary and stem cells. The … WebAcute myeloid leukemia (AML) is a malignant disorder of the progenitor cells in myeloid hematopoiesis and represents a genetically heterogeneous cancer. The onset of AML is thought to require cooperation between active proliferation and defects in myeloid differentiation, which often results in chromosomal translocation (Gilliland et al., 2004).

WebJul 1, 2010 · pLVX-Puro Vector Information . PT4002-5 Catalog No. 632164. pLVX-Puro Vector Map and Multiple Cloning Site (MCS). Description. pLVX-Puro is an HIV-1-based, lentiviral expression vector. Lentiviral particles derived from . the vector allow you to express your gene of interest in virtually any cell type, even primary cells. WebNov 23, 2024 · Alcoholic steatohepatitis (ASH) is a critical stage leading to cirrhosis and end-stage liver disease. However, its pathogenesis is still far from clearly understood, and a treatment that is widely...

WebpCDH-CMV-mCherry-T2A-Puro (Plasmid #72264) Print Enlarge View all sequences Purpose Express mCherry and puromycin resistance gene under CMV promoter. 3rd gen lentiviral vector. Depositing Lab Kazuhiro …

WebAddgene inc mcherry egfp lc3 Mcherry Egfp Lc3, supplied by Addgene inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more pictures and names of phalaenopsis orchidsWebJan 11, 2024 · Nonalcoholic fatty liver disease (NAFLD), characterized by excess hepatic fat accumulation, inflammation and oxidative stress, has become a burgeoning worldwide epidemic liver disease, affecting approximately one-quarter of the entire global population ( Diehl and Day, 2024 ). pictures and names of singersWebDescription: LV-CMV-mCherry-Puro is pre-made lentivirus which expresses mCherry under the CMV promoter with co-expression of puromycin selection under PGK promoter. … top golf free membershipWebCatalog number: LV-EF1a-mCherry Description: Lentiviral control particles with EF1a promoter-driven expression of mCherry and SV40-puro, ultracentrifugation purified Skip … pictures and otherWebNov 23, 2024 · A The efficiency of HBLV-m-PDPK1-3xflag-mCherry-PURO detected by RT-qPCR ( n = 3). B Immunoblot of cell lysates. Quantification of the ratio of PDPK1/β-actin ( … pictures and names of skin rashesWebMar 18, 2024 · HBLV-EDNRA-HIS-mCherry-BSD, and HBLV-NR3C2-3xflag- ... HBLV-ZsGreen-PURO and HBLV-mCherry-BSD empty. vectors were used as negative controls. Lentiviral transfection. pictures and names of weedsWebJan 30, 2024 · Gli1 overexpressed was conducted in MDA-MB-231 cells by lentivirus vector HBLV-h-Gli1-3xflag-ZsGreen-PURO. RT-qPCR and Western blot were detected the mRNA and protein level of genes of Hh pathway, Epithelial-mesenchymal transition (EMT) and stemness. The nuclear localization and expression of Gli1 was observed by laser … topgolf fresno